human bone marrow mesenchymal stem cells  (PromoCell)

Journal: Polymers

Article Title: Osteoinductive and Biocompatibility Assessment of a 3D-Printed Polymeric–Hydroxyapatite Composite Interference Screw

doi: 10.3390/polym18101239

Figure Lengend Snippet: Scanning electron microscopy characterization of mesenchymal stem cell attachment and proliferation on three biomaterial compositions. Top panels ( A – C ): Biomaterial surfaces prior to cell seeding, showing the characteristic surface morphology of Group 1 (PCL + HA, 60/40) ( A ), Group 2 (PCL + PLGA, 50/50) ( B ), and Group 3 (PCL + PLGA + HA, 30/30/40) ( C ) respectively. Bottom panels ( D – F ): Mesenchymal stem cells after 21 days of culture in osteogenic differentiation medium, demonstrating robust cell attachment, spreading, and surface colonization on Groups 1 ( D ), 2 ( E ), and 3 ( F ), respectively. Panel ( G ): Higher magnification view (×3500) illustrating characteristic elongated morphology of healthy, proliferative mesenchymal stem cells with extensive filopodia and lamellipodia formation extending across the Group 3 biomaterial surface, indicating strong cell–substrate interactions and active synthetic activity.

Article Snippet: Human bone marrow-derived mesenchymal stem cells were obtained from PromoCell (Heidelberg, Germany) and cultured in Mesenchymal Stem Cell Growth Medium MSC2 according to the manufacturer’s instructions.

Techniques: Electron Microscopy, Cell Attachment Assay, Activity Assay

Journal: Polymers

Article Title: Osteoinductive and Biocompatibility Assessment of a 3D-Printed Polymeric–Hydroxyapatite Composite Interference Screw

doi: 10.3390/polym18101239

Figure Lengend Snippet: Alamar Blue assay evaluating mesenchymal stem cell metabolic activity on three 3D-printed biomaterial compositions over time. ( A ) Line graph showing percentage of cell metabolic activity measured at days 3, 7, 14, and 21 on PCL + HA (60/40), PCL + PLGA (50/50), and PCL + PLGA + HA (30/30/40) substrates. Days 3, 7, 14, and 21 refer to the duration of osteogenic differentiation culture, defined as Day 0 of osteogenic induction initiated after 3 days of proliferation medium pre-culture. ( B ) Corresponding bar graph representation of metabolic activity for each composition at individual time points (D3, D7, D14, D21). Data are presented as mean ± SEM (n = 3 biological replicates per group per timepoint). Statistical annotations indicate comparisons between material groups as follows: * comparison between PCL + PLGA + HA and PCL + HA; # comparison between PCL + PLGA + HA and PCL + PLGA in A. Normality of residuals was assessed using the Shapiro–Wilk test, and homogeneity of variances was assessed using Levene’s test; assumptions were met for all groups (Shapiro p > 0.05; Levene p > 0.05). Statistical differences were evaluated by two-way ANOVA, followed by Tukey multiple comparisons with Bonferroni correction.#: PCL + PLGA + HA vs. PCL + PLGA (# p < 0.05, ## p < 0.01, (n = 3 per group)) *: PCL + PLGA + HA vs. PCL + HA ( p < 0.05 (*), p < 0.01 (**), p < 0.001 (***)).

Article Snippet: Human bone marrow-derived mesenchymal stem cells were obtained from PromoCell (Heidelberg, Germany) and cultured in Mesenchymal Stem Cell Growth Medium MSC2 according to the manufacturer’s instructions.

Techniques: Alamar Blue Assay, Activity Assay, Comparison

Journal: Polymers

Article Title: Osteoinductive and Biocompatibility Assessment of a 3D-Printed Polymeric–Hydroxyapatite Composite Interference Screw

doi: 10.3390/polym18101239

Figure Lengend Snippet: Relative gene expression of osteogenic markers in MSCs cultured on Implant A and Implant B 3D-printed PCL + PLGA + HA (30/30/40) composite scaffolds at days 14 and 21 of osteogenic differentiation. Days 14 and 21 refer to the duration of osteogenic differentiation culture, initiated after 3 days of proliferation medium pre-culture. Implant A: surface-coated PCL + PLGA + HA (30/30/40); Implant B: uncoated PCL + PLGA + HA (30/30/40) control. ( A ) Alkaline phosphatase (ALP), ( B ) Runt-related transcription factor 2 (RUNX2), and ( C ) Bone gamma-carboxyglutamate protein (BGLAP). Gene expression was normalized to GAPDH using the 2 −ΔΔCt method. Data are presented as mean ± SD (n = 4 biological replicates, each analyzed with three technical replicates). Normality of residuals was assessed using the Shapiro–Wilk test, and homogeneity of variances was assessed using Levene’s test; assumptions were met for all genes (Shapiro p > 0.05; Levene p > 0.05). Statistical differences were evaluated gene-by-gene using a two-way ANOVA (Time_Point: Day 14 vs. Day 21; Condition: Implant A vs. Implant B) including the interaction term (Time_Point × Condition), followed by Tukey/emmeans post hoc multiple comparisons with Bonferroni correction. Significance is indicated as follows: p < 0.05 (*) and p < 0.001 (***).

Article Snippet: Human bone marrow-derived mesenchymal stem cells were obtained from PromoCell (Heidelberg, Germany) and cultured in Mesenchymal Stem Cell Growth Medium MSC2 according to the manufacturer’s instructions.

Techniques: Gene Expression, Cell Culture, Control

Journal: Regenerative Therapy

Article Title: Anti-inflammatory and immunomodulatory effects of human induced pluripotent stem cells-derived mesenchymal stem/stromal cells and their extracellular vesicles

doi: 10.1016/j.reth.2026.101081

Figure Lengend Snippet: Effects of XF-iMSCs in mouse peripheral blood mononuclear cells (mPBMCs). mPBMCs (1 × 10 6 cells/mL) were stimulated with LPS (10 ng/mL) for 24 h, and levels of IL-6, TNF-α, and IL-10 in culture supernatants were quantified by ELISA. XF-iMSCs or human adipocyte-derived MSCs (hAC-MSCs) were co-cultured at a density of 2 × 10 5 cells/well with mPBMCs. Data are presented as mean ± SD. ∗∗∗ p < 0.001 (Student's t -test), ## p < 0.01; ### p < 0.001 (Dunnett test, vs control group).

Article Snippet: Human MSCs (adipocyte-derived MSCs, hAC-MSCs; bone marrow-derived MSCs, hBM-MSCs; umbilical cord-derived MSCs, hUC-MSCs) were purchased from PromoCell (Heidelberg, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, Cell Culture, Control

Journal: Regenerative Therapy

Article Title: Anti-inflammatory and immunomodulatory effects of human induced pluripotent stem cells-derived mesenchymal stem/stromal cells and their extracellular vesicles

doi: 10.1016/j.reth.2026.101081

Figure Lengend Snippet: Effects of XF-iMSCs in primary human PBMCs. uman peripheral blood mononuclear cells (hPBMCs, 2 × 10 6 cells/mL) were stimulated with LPS (10 ng/mL) for 24 h, and levels of IL-6, TNF-α, and IL-10 in culture supernatants were quantified by ELISA. The hPBMCs were cultured with human adipocyte-derived MSCs (hAC-MSCs), bone marrow-derived MSCs (hBM-MSCs), or umbilical cord-derived MSCs at a density of 2 × 10 5 cells/well. Data are presented as mean ± SD. +++ p < 0.001 (Aspin–Welch's t -test). ## p < 0.01; ### p < 0.001 (Dunnett test).

Article Snippet: Human MSCs (adipocyte-derived MSCs, hAC-MSCs; bone marrow-derived MSCs, hBM-MSCs; umbilical cord-derived MSCs, hUC-MSCs) were purchased from PromoCell (Heidelberg, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Derivative Assay

Journal: Regenerative Therapy

Article Title: Anti-inflammatory and immunomodulatory effects of human induced pluripotent stem cells-derived mesenchymal stem/stromal cells and their extracellular vesicles

doi: 10.1016/j.reth.2026.101081

Figure Lengend Snippet: Immunomodulatory effects of XF-iMSCs. MSC-mediated suppression of effector T cell (Teff) proliferation in CellTrace Violet (CTV)-labeled human PBMCs (hPBMCs) was evaluated by suppression assay. CTV-labeled hPBMCs (5 × 10 4 cells) were seeded as responder cells on Treg suppression inhibitor beads. XF-iMSCs or hAC-MSCs were co-cultured with CTV-labeled hPBMCs at different ratios (hPBMCs: MSCs = 1:0.5, 0.2, 0.1, and 0.05). The cells were harvested after 4 days of co-culture and analyzed for the proliferation of CTV-labeled effector T cells using flow cytometry. Data are presented as % inhibition. Stim, stimulation; No stim, no stimulation.

Article Snippet: Human MSCs (adipocyte-derived MSCs, hAC-MSCs; bone marrow-derived MSCs, hBM-MSCs; umbilical cord-derived MSCs, hUC-MSCs) were purchased from PromoCell (Heidelberg, Germany).

Techniques: Labeling, Suppression Assay, Cell Culture, Co-Culture Assay, Flow Cytometry, Inhibition

Journal: Materials

Article Title: Do Collagenated Xenogenic Bone Substitutes Enhance Gingival Healing and Angiogenesis Through a Barrier Membrane? An In Vitro Study

doi: 10.3390/ma19040680

Figure Lengend Snippet: ( A ) BMP-2 secretion by human gingival cells. ELISA analysis showed that none of the tested materials affected BMP-2 secretion. ( B ) Effects of the materials on human mesenchymal stem cell migration assessed using Boyden chambers. ( a – d ) Representative images used for quantification of migrating cells: ( a ) control, ( b ) Bio-Oss, ( c ) Gen-Os, and ( d ) GTO (scale bar = 50 µm). ( e ) Gen-Os and GTO significantly increased hMSC migration compared with the control and Bio-Oss. Data represent four independent biological replicates. Different lowercase letters indicate statistically significant differences among groups (Tukey’s post hoc test, p < 0.05).

Article Snippet: Human mesenchymal stem cells derived from bone marrow (hMSCs) were obtained from PromoCell (Heidelberg, Germany) and cultured in Mesenchymal Growth Medium 2 (MGM-2; PromoCell) supplemented with SupplementMix at 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Enzyme-linked Immunosorbent Assay, Migration, Control

Journal: Materials

Article Title: Do Collagenated Xenogenic Bone Substitutes Enhance Gingival Healing and Angiogenesis Through a Barrier Membrane? An In Vitro Study

doi: 10.3390/ma19040680

Figure Lengend Snippet: Schematic representation of the interaction between bone grafting materials and gingival cells. Tooth extraction results in alveolar bone resorption. The ridge preservation technique involves placement of a bone grafting material into the extraction socket to limit the bone resorption process. Following application, material extracts interact with injured gingival cells through the collagen membrane (1), inducing cell proliferation (2). In response, gingival cells secrete growth factors (3), which promote neoangiogenesis (4) and mesenchymal stem cell recruitment (5).

Article Snippet: Human mesenchymal stem cells derived from bone marrow (hMSCs) were obtained from PromoCell (Heidelberg, Germany) and cultured in Mesenchymal Growth Medium 2 (MGM-2; PromoCell) supplemented with SupplementMix at 37 °C in a humidified atmosphere of 5% CO 2 .

Techniques: Extraction, Preserving, Membrane